Differential expression of pinoresinol-lariciresinol reductase gene in relation to podophyllotoxin accumulation in different plant organs of endangered anticancer species Podophyllum peltatum

Pinoresinol-lariciresinol reductase (PLR) is a key enzyme in podophyllotoxin (PTOX) biosynthesis pathway, which catalyzes the conversion of pinoresinol into secoisolariciresinol, central precursor for PTOX biosynthesis. Podophyllum peltatum an important anticancer species, and content obviously different from various plant organs. Therefore, investigation PLR gene expression facilitates understanding molecular mechanisms variation organs P. as well its mechanisms, suggesting that it metabolic engineering PTOX. The was found all test (root, rhizome, petiole, fruit, leaf flower), with significant differences among them (p<0.05). highest (2.8018 ± 0.23%) 30 times higher than lowest fruit. Furthermore, tissue profile showed (PpPLR) expressed except flower. level rhizome leaf, respectively. PpPLR inconsistent biosynthetic organ not necessarily storage organ. After initial synthesis, can be transferred to organ, such rhizome.